caspase dependent apoptosis induced by cladribine in the estrogen receptor negative breast cancer cell line, mda-mb468
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abstract
the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apoptosis. the activation of caspase-3 and -9 was assayed using caspase activation assay kits. gel electrophoresis was performed to detect dna fragmentation. treatment of mda-mb468 cells with different concentrations of 2-cda (50, 100 and 500 µm) resulted in a significant increase in the cell death. annexin v-fluorescein/pi and hoechst 33258 staining revealed that the cell death was mainly of apoptotic type. dna laddering profile was also obtained in the treated mda-mb468 cells using dna fragmentation analysis. a significant (p
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Journal title:
journal of sciences islamic republic of iranجلد ۱۴، شماره ۴، صفحات ۳۰۳-۳۱۰
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